超清版 GB/T 44293-2024 显微物镜数值孔径图像式测量方法

ICS 31.260
CCS L 50
中华人民共和国国家标准
GB/T 44293—2024
显微物镜数值孔径图像式测量方法
Image measurement method for numerical aperture of microscopic objective

2024-08-23 发布2025-03-01 实施
国家市场监督管理总局
国家标准化管理委员会发 布

目　　次
前言 ····································································································· Ⅲ
1 范围 ·································································································· 1
2 规范性引用文件 ······················································································ 1
3 术语和定义 ··························································································· 1
4 测量原理 ······························································································ 2
5 测量装置 ······························································································ 4
5.1 装置组成 ························································································· 4
5.2 成像照明模块 ···················································································· 4
5.3 调焦模块 ························································································· 4
5.4 测量照明模块 ···················································································· 5
5.5 测量模块 ························································································· 5
5.6 探测模块 ························································································· 5
5.7 数据处理模块 ···················································································· 5
6 测量环境 ······························································································ 5
7 测量步骤 ······························································································ 5
8 不确定度分析 ························································································· 6
附录A （规范性） 数值孔径圆直径和全反射圆直径计算方法 ········································ 8
附录B （资料性） 显微物镜数值孔径测量结果记录表 ··············································· 10
附录C （资料性） NA1.50 显微物镜数值孔径测量不确定度分析 ··································· 11
参考文献 ································································································ 12

前　　言
本文件按照GB/T 1.1—2020《标准化工作导则　第1 部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由中国科学院提出。
本文件由全国光电测量标准化技术委员会（SAC/TC 487）和全国光学和光子学标准化技术委员会
（SAC/TC 103）联合归口。
本文件起草单位：中国科学院苏州生物医学工程技术研究所、齐之明光电智能科技（苏州）有限公
司、苏州国科医工科技发展（集团）有限公司、苏州市计量测试院、中国计量科学研究院、中国科学院
空天信息创新研究院、中国计量大学、宁波舜宇仪器有限公司、宁波永新光学股份有限公司、南京智群
光电技术有限公司、之江实验室、南京施密特光学仪器有限公司。
本文件主要起草人：巩岩、唐玉国、张艳微、郎松、黎俊、徐英莹、王璞、占春连、胡森虎、
崔志英、袁群、张建锋、雷震。


显微物镜数值孔径图像式测量方法
1　范围
本文件描述了显微物镜数值孔径图像式测量方法的测量原理、测量装置、测量环境、测量步骤以及
不确定度分析。
本文件适用于数值孔径标称值大于1.00 的无限远校正显微物镜的数值孔径测量 。
2　规范性引用文件
下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文