超清版 GB/T 44214-2024 蚕丝中非蚕丝蛋白物质含量试验方法

ICS 59.080.01
CCS W 40
中华人民共和国国家标准
GB/T 44214—2024
蚕丝中非蚕丝蛋白物质含量试验方法
Test method for non-protein content in silk
2024-07-24 发布2025-02-01 实施
国家市场监督管理总局
国家标准化管理委员会发 布

目　　次
前言 ····································································································· Ⅲ
1 范围 ·································································································· 1
2 规范性引用文件 ······················································································ 1
3 术语和定义 ··························································································· 1
4 原理 ·································································································· 1
5 试剂和材料 ··························································································· 1
6 仪器和设备 ··························································································· 2
7 试验步骤 ······························································································ 2
7.1 标准溶液配制 ···················································································· 2
7.2 缓冲液配制 ······················································································ 2
7.3 样品前处理 ······················································································ 3
7.4 衍生化操作 ······················································································ 3
7.5 萃取净化 ························································································· 3
7.6 测定 ······························································································ 3
8 结果计算与表示 ······················································································ 4
8.1 蚕丝中氨基酸含量分析 ·········································································· 4
8.2 非蚕丝蛋白物质含量分析 ········································································ 5
9 精密度 ································································································ 5
10 试验报告 ···························································································· 5
附录A （资料性） 四种氨基酸化合物信息 ···························································· 6
GB/T 44214—2024
Ⅰ

前　　言
本文件按照GB/T 1.1—2020《标准化工作导则　第1 部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由中国纺织工业联合会提出。
本文件由全国丝绸标准化技术委员会（SAC/TC 401）归口。
本文件起草单位：浙江丝绸科技有限公司、黄山市信达丝线有限公司、浙江蚕缘家纺股份有限公
司、苏州太湖雪丝绸股份有限公司、嵊州陌桑高科股份有限公司、苏州大学、苏州市纤维检验院、南通
市纤维检验所、浙江巴贝领带有限公司、达利丝绸（浙江）有限公司、南充银海丝绸有限公司、四川安
泰茧丝绸集团有限公司、浙江省检验检疫科学技术研究院、杭州市质量技术监督检测院、上海市质量监
督检验技术研究院、浙江生态纺织品禁用染化料检测中心有限公司、江苏工程职业技术学院、中芯创惟
生物科技(杭州) 有限公司、苏州市知识产权保护中心、绍兴市柯桥区中国轻纺城检验检测与标准化研
究院。
本文件主要起草人：吴国坚、伍冬平、陈思宇、孙晨晓、祁宁、刘雨、江朝辉、赵海浪、陈凤鸣、
刘伟、雷斌、倪学林、金丰、何国君、文朝钧、朱杨锐、杭志伟、李婷、曹石淼、陈志华、尹桂波、
潘璐璐、蒋伟、冯桂华、童珈珈。
GB/T 44214—2024