超清版 GB/T 44613-2024 牛羊副流感病毒3型诊断技术

牛羊副流感病毒3 型诊断技术
Diagnostic techniques for bovine and caprine parainfluenza virus type 3
ICS 11.220
CCS B 41
中华人民共和国国家标准
GB/T 44613—2024
2024-09-29 发布2025-04-01 实施
国家市场监督管理总局
国家标准化管理委员会发 布

目　　次
前言 ····································································································· Ⅲ
引言 ····································································································· Ⅳ
1 范围 ·································································································· 1
2 规范性引用文件 ······················································································ 1
3 术语和定义 ··························································································· 1
4 缩略语 ································································································ 1
5 临床诊断 ······························································································ 2
6 样品采集、处理与保存 ··············································································· 2
7 病毒分离鉴定 ························································································· 4
8 RT﹘PCR 试验 ························································································· 5
9 Real﹘time RT﹘PCR 试验 ··············································································· 6
10 cELISA 试验 ························································································· 7
11 病毒中和试验 ······················································································· 9
12 综合判定 ··························································································· 11
附录 A （规范性）　试剂配制 ·········································································· 12
附录 B （规范性）　病毒核酸检测用引物和荧光探针序列 ············································ 13
附录 C （规范性）　RT﹘PCR 和 Real﹘time RT﹘PCR 用阳性对照品制备、引物及序列 ················ 14
附录 D （规范性）　RT﹘PCR 和 Real﹘time RT﹘PCR 反应体系及反应程序 ···························· 16
附录 E （资料性）　RT﹘PCR 和Real﹘time RT﹘PCR 试验结果图 ······································ 18
附录 F （资料性）　病毒标准阳性血清、标准阴性血清和抗原包被酶标板的制备 ···················· 20
附录 G （资料性）　酶标单克隆抗体的制备 ··························································· 21
GB/T 44613—2024
Ⅰ

前　　言
本文件按照GB/T 1.1—2020 《标准化工作导则　第 1 部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由中华人民共和国农业农村部提出。
本文件由全国动物卫生标准化技术委员会（SAC/TC 181）归口。
本文件起草单位：吉林农业科技学院、江苏省农业科学院、吉林省动物疫病预防控制中心、吉林大
学、中国动物卫生与流行病学中心。
本文件主要起草人：郭利、孙亮、李文良、张金玉、宋建德、刘茂军、杨蕾蕾、毛立、李基棕、
程悦宁、郝飞、孙敏、王楠、王振军、孙娜、冯晓宇。
GB/T 44613—2024
Ⅲ
引　　言
牛副流感病毒 3 型（Bovine parainfluenza virus type 3，BPIV3）和山羊副流感病毒 3 型（Caprine
parainfluenza virus type 3，CPIV3）同属于副黏病毒科（Paramyxoviridae）副黏病毒亚科（Paramyx﹘
ovirinae）呼吸道病毒属（Respirovirus），两者核苷酸同源性接近 81%，血清学有交叉反应。2022
年，国际病毒分类委员会（Internat