超清版 GB/T 44482-2024 化学品 嗜热四膜虫多代繁殖毒性试验

ICS 13.300
CCS R 04
中华人民共和国国家标准
GB/T 44482—2024
化学品　嗜热四膜虫多代繁殖毒性试验
Chemicals—Tetrahymena thermophila multi-generational reproductive test
2024-09-29 发布2025-04-01 实施
国家市场监督管理总局
国家标准化管理委员会发 布

目　　次
前言 ····································································································· Ⅲ
1 范围 ·································································································· 1
2 规范性引用文件 ······················································································ 1
3 术语和定义 ··························································································· 1
4 试验原理 ······························································································ 1
5 受试物信息 ··························································································· 2
6 参比物质 ······························································································ 2
7 试验系统 ······························································································ 2
7.1 仪器设备 ························································································· 2
7.2 材料和试剂 ······················································································ 2
7.3 受试生物的选取 ················································································· 3
7.4 试验溶液 ························································································· 3
7.5 试验前的准备 ···················································································· 3
7.6 预试验 ··························································································· 3
7.7 正式试验程序 ···················································································· 3
8 限度试验 ······························································································ 4
9 质量控制 ······························································································ 5
10 数据与报告 ·························································································· 5
10.1 抑制率的计算 ··················································································· 5
10.2 ECx 的计算 ······················································································ 5
10.3 LOEC 和NOEC 的计算 ········································································· 5
10.4 试验报告 ························································································ 5
附录 A （资料性）　嗜热四膜虫的实验室维护及培养方法实施指南 ··································· 7
A.1 概述 ····························································································· 7
A.2 常用培养基 ······················································································ 7
A.3 嗜热四膜虫的保种 ·············································································· 7
A.4 嗜热四膜虫的培养 ···