GB/T 45105-2024 绵羊痘和山羊痘诊断技术

中华人民共和国国家标准
ICS 11.220
CCS B 41
GB/T 45105—2024
绵羊痘和山羊痘诊断技术
Diagnostic techniques for sheep pox and goat pox
2024⁃12⁃31 发布2025⁃07⁃01 实施
国家市场监督管理总局
国家标准化管理委员会
发 布

GB/T 45105—2024
目　　次
前言··························································································································Ⅲ
引言··························································································································Ⅳ
1　范围·······················································································································1
2　规范性引用文件········································································································1
3　术语和定义··············································································································1
4　缩略语····················································································································1
5　临床诊断·················································································································1
6　样品采集、保存与运输································································································2
7　病毒分离与鉴定········································································································3
8　组织学检查··············································································································4
9　PCR 检测················································································································4
10　实时荧光PCR 检测··································································································6
11　LAMP 检测············································································································7
12　病毒中和试验·········································································································8
13　ELISA··················································································································9
14　综合判定··············································································································10
附录A（规范性）　常用试剂溶液配制················································································11
附录B（规范性）　PCR 溶液的配制··················································································12
附录C（资料性）　CaPV PCR 检测结果对照·······································································15
附录D（资料性）　CaPV 实时荧光PCR 检测结果对照··························································16
附录E（资料性）　CaPV LAMP 检测结果参照图·································································17
附录F（资料性）　Reed⁃Muench 法计算中和抗体效价···························································18
附录G（资料性）　CaPV 重组P32 蛋白抗原的制备······························································19
附录H（资料性）　阴性对照和阳性对照的制备·························