GB/T 45238-2025 化学品 用虹鳟肝S9亚细胞组分测定体外固有清除率试验

中华人民共和国国家标准
ICS 13.300
CCS A 80
GB/T 45238—2025
化学品　用虹鳟肝S9 亚细胞组分测定
体外固有清除率试验
Chemicals—Determination of in vitro intrinsic clearance using rainbow trout
liver S9 sub⁃cellular fraction（ RT⁃S9）
2025⁃01⁃24 发布2025⁃05⁃01 实施
国家市场监督管理总局
国家标准化管理委员会
发 布

GB/T 45238—2025
目　　次
前言··························································································································Ⅲ
引言··························································································································Ⅳ
1　范围·······················································································································1
2　规范性引用文件········································································································1
3　术语和定义、符号和缩略语··························································································1
4　试验原理·················································································································3
5　受试物信息··············································································································3
6　参比物····················································································································4
7　试验准备·················································································································4
8　试验程序·················································································································5
9　数据处理·················································································································6
10　质量保证与质量控制· ······························································································7
11　结果报告···············································································································7
附录A（资料性）　虹鳟肝S9 亚细胞组分的制备···································································9
附录B（资料性）　虹鳟肝亚细胞组分的表征·······································································16
附录C（资料性）　酶促失活的虹鳟肝S9 亚细胞组分的制备···················································17
附录D（资料性）　初步试验反应条件的建立·······································································18
附录E（资料性）　试验方法的建立···················································································20
附录F（资料性）　缓冲溶液、辅因子、丙甲甘肽溶液的配制和RT⁃S9 的稀释· ·····························22
参考文献····················································································································25
Ⅰ

GB/T 45238—2025
前 言
本文件按照GB/T 1.1—2020《标准化工作导则　第1 部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由全国危险化学品管理标准化技术委员会（SAC/TC 251）提出并归口。
本文件起草单位：广东省科学院微生物研究所（广东省微生物分析检测中心）、生态环境部固体废
物与化学品管理技术中心、中检科健（天津）检验检测有限责任公司、中国水产科学研究院珠江水产研
究所。
本文件主要起草人：梅承芳、许玫英、陈桂兰、王青柏、陈会明、窦从从、